aav9 hsyn jgcamp7b Search Results


aav9- hsyn1- chi[mouse(shcdk5)]-jgcamp7b -wpre- sv40p(a)  (CH Instruments)
90
CH Instruments aav9- hsyn1- chi[mouse(shcdk5)]-jgcamp7b -wpre- sv40p(a)
Aav9 Hsyn1 Chi[Mouse(shcdk5)] Jgcamp7b Wpre Sv40p(a), supplied by CH Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aav9+hsyn+jgcamp7b/aav9++hsyn1++chi+mouse+shcdk5+++jgcamp7b++wpre++sv40p+a+/10__7554_slash_elife__97029-528-18-20
Average 90 stars, based on 1 article reviews
aav9- hsyn1- chi[mouse(shcdk5)]-jgcamp7b -wpre- sv40p(a) - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
aav9 hsyn jgcamp7b  (Addgene inc)
93
Addgene inc aav9 hsyn jgcamp7b
a. Schematic of longitudinal Ca 2+ imaging recording schedules from the SoD experience session to the CFC memory recall session. b. Expression of the Janelia variant Ca <t>2+</t> <t>indicator</t> <t>AAV9-hSyn-JGCaMP7b</t> and the location of the implanted GRIN lens in the CA1 region of the dorsal hippocampus. Scale bar, 250 µm. c. Example image of cells extracted from Ca 2+ imaging and representative traces of z scored neural activity. d. Schematic of the analysis flowchart for detecting recall-specific ensembles using PCA/ICA dimensionality reduction methods. After all PCA/ICA ensembles from the CFC recall session were detected, these ensembles were further categorized into recall-specific (pink) or recall-nonspecific ensembles (blue) by comparing the ensemble activity in the CFC recall session with that in the previous CFC pre-shock session by Wilcoxon rank sum methods. For each example trace of the ensemble activity, the corresponding raster plot of the neural activity is shown with the same colour. e. Multisession comparison of the averaged activity index of the recall-specific ensembles, which is normalized by the average activity rate of the recall-nonspecific ensembles per mouse for each corresponding session. (Neutral n = 14; SoD n = 28 ensembles; two-way RM ANOVA with correction; interaction P <0.001; F (6, 240) = 5.382; Šídák’s multiple comparisons test). f. Pearson correlation analysis of the activity index of recall-specific ensemble during the SI-ICR session and prospective total freezing level (%) in the CFC recall session per SoD mouse. ( r = 0.9378, P = 0.0184, n = 5 mice) g. Activity rate of each CFC recall-specific ensemble in the neutral group in the home cage before the neutral experience (Pre-Neutral-home cage) and during the neutral session (novel home cage exploration) on day 6 ( n = 14 ensembles; Wilcoxon signed rank test; P = 0.3306); additionally, the activity rate of the SoD group in the home cage before the SoD session (Pre-SoD-home cage) and during the SoD session on day 6 of the SoD experience ( n = 28 ensembles, Wilcoxon signed rank test, P = 0.0014). h. Activity rate of each recall-specific ensemble during the novel B6 mouse encounter (SI-B6) and novel ICR mouse encounter (SI-ICR) in the social interaction test for the neutral group ( n = 14 ensembles, Wilcoxon signed rank test, P = 0.4352) and SoD group ( n = 28 ensembles, Wilcoxon signed rank test, P = 0.0066). i. Activity rate of each recall-specific ensemble during the 2-minute exploration period before receiving the first electric shock (CFC pre-shock) and during the remaining session after the delivery of the first shock (CFC post-shock) on the CFC conditioning day in the neutral group ( n = 14 ensembles; Wilcoxon signed rank test; P = 0.0076) and the SoD group ( n = 28 ensembles, Wilcoxon signed rank test, P = 0.0053). j. Schematic for assessing the correlation of the CFC recall-specific ensemble activity during opposite corner stays during the SI-ICR session and the ensemble activity during the freeze state in the CFC recall session. Example traces of averaged recall-specific ensemble activity (z scored) around the onset of the opposite corner entries in the SI-ICR (lower left) and during freezing behaviours in CFC recall (lower right) sessions. k. Spearman correlation ( r value shown) for the activity of the CFC recall-specific ensembles in the neutral group during opposite corner stays and activity during freezing in the CFC recall session for the neutral group ( r = - 0.3600, P = 0.2047) and l. SoD group ( r = 0.4426, P = 0.0208, n = 27 ensembles). m. Representative heatmaps showing the intercell type pairwise correlation analysis between SI avoidance-related (SI opposite corner-related neurons) and CFC aversion-related (shock- and freeze-related neurons) cells in the non-freezing state (left) and the freezing state in the CFC recall session (right). Red indicates a significant correlation between two neurons. n. Percentage of significant pairwise correlations of SI avoidance-related and CFC aversion-related cells during the freezing state in the CFC recall session (Neutral n = 5; SoD n = 5 mice; unpaired t test, t = 2.653; P = 0.0291). o. Percentage of significant intracell type neuron‒neuron pairwise correlations of SI avoidance-related neurons during the freezing state in the CFC recall session (Neutral, n = 5; SoD, n = 5 mice; Unpaired t test, t = 0.2808; P = 0.786). Data are presented as the mean ± s.e.m. Statistical significance is expressed as * P < 0.05; ** P < 0.01; and *** P < 0.001.
Aav9 Hsyn Jgcamp7b, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aav9+hsyn+jgcamp7b/pGP-AAV-syn-jGCaMP7b-WPRE+(Plasmid+%23104489)/bio_rxiv__64898__2026__03__03__709197-170-15-18
Average 93 stars, based on 1 article reviews
aav9 hsyn jgcamp7b - by Bioz Stars, 2026-10
93/100 stars
  Buy from Supplier
aav9 hsyn1 flex jgcamp7b  (Addgene inc)
93
Addgene inc aav9 hsyn1 flex jgcamp7b
a. Schematic of longitudinal Ca 2+ imaging recording schedules from the SoD experience session to the CFC memory recall session. b. Expression of the Janelia variant Ca <t>2+</t> <t>indicator</t> <t>AAV9-hSyn-JGCaMP7b</t> and the location of the implanted GRIN lens in the CA1 region of the dorsal hippocampus. Scale bar, 250 µm. c. Example image of cells extracted from Ca 2+ imaging and representative traces of z scored neural activity. d. Schematic of the analysis flowchart for detecting recall-specific ensembles using PCA/ICA dimensionality reduction methods. After all PCA/ICA ensembles from the CFC recall session were detected, these ensembles were further categorized into recall-specific (pink) or recall-nonspecific ensembles (blue) by comparing the ensemble activity in the CFC recall session with that in the previous CFC pre-shock session by Wilcoxon rank sum methods. For each example trace of the ensemble activity, the corresponding raster plot of the neural activity is shown with the same colour. e. Multisession comparison of the averaged activity index of the recall-specific ensembles, which is normalized by the average activity rate of the recall-nonspecific ensembles per mouse for each corresponding session. (Neutral n = 14; SoD n = 28 ensembles; two-way RM ANOVA with correction; interaction P <0.001; F (6, 240) = 5.382; Šídák’s multiple comparisons test). f. Pearson correlation analysis of the activity index of recall-specific ensemble during the SI-ICR session and prospective total freezing level (%) in the CFC recall session per SoD mouse. ( r = 0.9378, P = 0.0184, n = 5 mice) g. Activity rate of each CFC recall-specific ensemble in the neutral group in the home cage before the neutral experience (Pre-Neutral-home cage) and during the neutral session (novel home cage exploration) on day 6 ( n = 14 ensembles; Wilcoxon signed rank test; P = 0.3306); additionally, the activity rate of the SoD group in the home cage before the SoD session (Pre-SoD-home cage) and during the SoD session on day 6 of the SoD experience ( n = 28 ensembles, Wilcoxon signed rank test, P = 0.0014). h. Activity rate of each recall-specific ensemble during the novel B6 mouse encounter (SI-B6) and novel ICR mouse encounter (SI-ICR) in the social interaction test for the neutral group ( n = 14 ensembles, Wilcoxon signed rank test, P = 0.4352) and SoD group ( n = 28 ensembles, Wilcoxon signed rank test, P = 0.0066). i. Activity rate of each recall-specific ensemble during the 2-minute exploration period before receiving the first electric shock (CFC pre-shock) and during the remaining session after the delivery of the first shock (CFC post-shock) on the CFC conditioning day in the neutral group ( n = 14 ensembles; Wilcoxon signed rank test; P = 0.0076) and the SoD group ( n = 28 ensembles, Wilcoxon signed rank test, P = 0.0053). j. Schematic for assessing the correlation of the CFC recall-specific ensemble activity during opposite corner stays during the SI-ICR session and the ensemble activity during the freeze state in the CFC recall session. Example traces of averaged recall-specific ensemble activity (z scored) around the onset of the opposite corner entries in the SI-ICR (lower left) and during freezing behaviours in CFC recall (lower right) sessions. k. Spearman correlation ( r value shown) for the activity of the CFC recall-specific ensembles in the neutral group during opposite corner stays and activity during freezing in the CFC recall session for the neutral group ( r = - 0.3600, P = 0.2047) and l. SoD group ( r = 0.4426, P = 0.0208, n = 27 ensembles). m. Representative heatmaps showing the intercell type pairwise correlation analysis between SI avoidance-related (SI opposite corner-related neurons) and CFC aversion-related (shock- and freeze-related neurons) cells in the non-freezing state (left) and the freezing state in the CFC recall session (right). Red indicates a significant correlation between two neurons. n. Percentage of significant pairwise correlations of SI avoidance-related and CFC aversion-related cells during the freezing state in the CFC recall session (Neutral n = 5; SoD n = 5 mice; unpaired t test, t = 2.653; P = 0.0291). o. Percentage of significant intracell type neuron‒neuron pairwise correlations of SI avoidance-related neurons during the freezing state in the CFC recall session (Neutral, n = 5; SoD, n = 5 mice; Unpaired t test, t = 0.2808; P = 0.786). Data are presented as the mean ± s.e.m. Statistical significance is expressed as * P < 0.05; ** P < 0.01; and *** P < 0.001.
Aav9 Hsyn1 Flex Jgcamp7b, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aav9+hsyn+jgcamp7b/pGP-AAV-syn-FLEX-jGCaMP7b-WPRE+(Plasmid+%23104493)/pmc10514716-4-0-2
Average 93 stars, based on 1 article reviews
aav9 hsyn1 flex jgcamp7b - by Bioz Stars, 2026-10
93/100 stars
  Buy from Supplier
low titer cre  (Addgene inc)
96
Addgene inc low titer cre
a. Schematic of longitudinal Ca 2+ imaging recording schedules from the SoD experience session to the CFC memory recall session. b. Expression of the Janelia variant Ca <t>2+</t> <t>indicator</t> <t>AAV9-hSyn-JGCaMP7b</t> and the location of the implanted GRIN lens in the CA1 region of the dorsal hippocampus. Scale bar, 250 µm. c. Example image of cells extracted from Ca 2+ imaging and representative traces of z scored neural activity. d. Schematic of the analysis flowchart for detecting recall-specific ensembles using PCA/ICA dimensionality reduction methods. After all PCA/ICA ensembles from the CFC recall session were detected, these ensembles were further categorized into recall-specific (pink) or recall-nonspecific ensembles (blue) by comparing the ensemble activity in the CFC recall session with that in the previous CFC pre-shock session by Wilcoxon rank sum methods. For each example trace of the ensemble activity, the corresponding raster plot of the neural activity is shown with the same colour. e. Multisession comparison of the averaged activity index of the recall-specific ensembles, which is normalized by the average activity rate of the recall-nonspecific ensembles per mouse for each corresponding session. (Neutral n = 14; SoD n = 28 ensembles; two-way RM ANOVA with correction; interaction P <0.001; F (6, 240) = 5.382; Šídák’s multiple comparisons test). f. Pearson correlation analysis of the activity index of recall-specific ensemble during the SI-ICR session and prospective total freezing level (%) in the CFC recall session per SoD mouse. ( r = 0.9378, P = 0.0184, n = 5 mice) g. Activity rate of each CFC recall-specific ensemble in the neutral group in the home cage before the neutral experience (Pre-Neutral-home cage) and during the neutral session (novel home cage exploration) on day 6 ( n = 14 ensembles; Wilcoxon signed rank test; P = 0.3306); additionally, the activity rate of the SoD group in the home cage before the SoD session (Pre-SoD-home cage) and during the SoD session on day 6 of the SoD experience ( n = 28 ensembles, Wilcoxon signed rank test, P = 0.0014). h. Activity rate of each recall-specific ensemble during the novel B6 mouse encounter (SI-B6) and novel ICR mouse encounter (SI-ICR) in the social interaction test for the neutral group ( n = 14 ensembles, Wilcoxon signed rank test, P = 0.4352) and SoD group ( n = 28 ensembles, Wilcoxon signed rank test, P = 0.0066). i. Activity rate of each recall-specific ensemble during the 2-minute exploration period before receiving the first electric shock (CFC pre-shock) and during the remaining session after the delivery of the first shock (CFC post-shock) on the CFC conditioning day in the neutral group ( n = 14 ensembles; Wilcoxon signed rank test; P = 0.0076) and the SoD group ( n = 28 ensembles, Wilcoxon signed rank test, P = 0.0053). j. Schematic for assessing the correlation of the CFC recall-specific ensemble activity during opposite corner stays during the SI-ICR session and the ensemble activity during the freeze state in the CFC recall session. Example traces of averaged recall-specific ensemble activity (z scored) around the onset of the opposite corner entries in the SI-ICR (lower left) and during freezing behaviours in CFC recall (lower right) sessions. k. Spearman correlation ( r value shown) for the activity of the CFC recall-specific ensembles in the neutral group during opposite corner stays and activity during freezing in the CFC recall session for the neutral group ( r = - 0.3600, P = 0.2047) and l. SoD group ( r = 0.4426, P = 0.0208, n = 27 ensembles). m. Representative heatmaps showing the intercell type pairwise correlation analysis between SI avoidance-related (SI opposite corner-related neurons) and CFC aversion-related (shock- and freeze-related neurons) cells in the non-freezing state (left) and the freezing state in the CFC recall session (right). Red indicates a significant correlation between two neurons. n. Percentage of significant pairwise correlations of SI avoidance-related and CFC aversion-related cells during the freezing state in the CFC recall session (Neutral n = 5; SoD n = 5 mice; unpaired t test, t = 2.653; P = 0.0291). o. Percentage of significant intracell type neuron‒neuron pairwise correlations of SI avoidance-related neurons during the freezing state in the CFC recall session (Neutral, n = 5; SoD, n = 5 mice; Unpaired t test, t = 0.2808; P = 0.786). Data are presented as the mean ± s.e.m. Statistical significance is expressed as * P < 0.05; ** P < 0.01; and *** P < 0.001.
Low Titer Cre, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aav9+hsyn+jgcamp7b/pENN%2EAAV%2EhSyn%2ECre%2EWPRE%2EhGH+(Plasmid+%23105553)/bio_rxiv__2022__02__16__480643-183-17-20
Average 96 stars, based on 1 article reviews
low titer cre - by Bioz Stars, 2026-10
96/100 stars
  Buy from Supplier
retro aav hsyn1 gcamp6s  (Addgene inc)
93
Addgene inc retro aav hsyn1 gcamp6s

Retro Aav Hsyn1 Gcamp6s, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aav9+hsyn+jgcamp7b/AAV-hSyn1-GCaMP6s-P2A-nls-dTomato+(Plasmid+%2351084)/pmc10514716-236-14-23
Average 93 stars, based on 1 article reviews
retro aav hsyn1 gcamp6s - by Bioz Stars, 2026-10
93/100 stars
  Buy from Supplier
aav9 camkiia gcamp6s addgene rrid addgene 107790 strain  (Addgene inc)
94
Addgene inc aav9 camkiia gcamp6s addgene rrid addgene 107790 strain

Aav9 Camkiia Gcamp6s Addgene Rrid Addgene 107790 Strain, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aav9+hsyn+jgcamp7b/AAV%2ECamKII%2EGCaMP6s%2EWPRE%2ESV40+(Plasmid+%23107790)/10__7554_slash_elife__100196-195-74-94
Average 94 stars, based on 1 article reviews
aav9 camkiia gcamp6s addgene rrid addgene 107790 strain - by Bioz Stars, 2026-10
94/100 stars
  Buy from Supplier
rene hen strain  (Addgene inc)
96
Addgene inc rene hen strain

Rene Hen Strain, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aav9+hsyn+jgcamp7b/pAAV%2ESyn%2EFlex%2EGCaMP6s%2EWPRE%2ESV40+(Plasmid+%23100845)/10__7554_slash_elife__100196-195-62-94
Average 96 stars, based on 1 article reviews
rene hen strain - by Bioz Stars, 2026-10
96/100 stars
  Buy from Supplier
aav9 camkii cre gfp addgene rrid addgene 105551 strain  (Addgene inc)
94
Addgene inc aav9 camkii cre gfp addgene rrid addgene 105551 strain

Aav9 Camkii Cre Gfp Addgene Rrid Addgene 105551 Strain, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aav9+hsyn+jgcamp7b/pENN%2EAAV%2ECamKII%2EHI%2EGFP-Cre%2EWPRE%2ESV40+(Plasmid+%23105551)/10__7554_slash_elife__100196-195-124-120
Average 94 stars, based on 1 article reviews
aav9 camkii cre gfp addgene rrid addgene 105551 strain - by Bioz Stars, 2026-10
94/100 stars
  Buy from Supplier
htr1a mouse rnascope probe advanced cell diagnostics  (Addgene inc)
92
Addgene inc htr1a mouse rnascope probe advanced cell diagnostics
Figure 6. Genetic knockdown of <t>Htr1a</t> receptors in dentate gyrus (DG) impairs infraslow oscillatory (ISO) and memory performance. (A) Schematic representation of the experimental design. A mix of AAV9-CamKII-GCaMP6s and AAV1-hSyn-Cre was injected into the DG of Htr1aflox/flox mice. (B) Representative example showing photometry and EEG recordings in the DG of a control mouse injected with AAV9-CaMKII-GCaMP6s alone. Right, Fourier transformation of calcium activity during wake (blue) non-rapid eye movement (NREM) sleep (red). (C) A representative example showing
Htr1a Mouse Rnascope Probe Advanced Cell Diagnostics, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aav9+hsyn+jgcamp7b/UbC+NLS-HA-MCP-YFP+(Plasmid+%2331230)/10__7554_slash_elife__100196-195-142-136
Average 92 stars, based on 1 article reviews
htr1a mouse rnascope probe advanced cell diagnostics - by Bioz Stars, 2026-10
92/100 stars
  Buy from Supplier
virus strains aav9  (Addgene inc)
91
Addgene inc virus strains aav9
Figure 6. Genetic knockdown of <t>Htr1a</t> receptors in dentate gyrus (DG) impairs infraslow oscillatory (ISO) and memory performance. (A) Schematic representation of the experimental design. A mix of AAV9-CamKII-GCaMP6s and AAV1-hSyn-Cre was injected into the DG of Htr1aflox/flox mice. (B) Representative example showing photometry and EEG recordings in the DG of a control mouse injected with AAV9-CaMKII-GCaMP6s alone. Right, Fourier transformation of calcium activity during wake (blue) non-rapid eye movement (NREM) sleep (red). (C) A representative example showing
Virus Strains Aav9, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aav9+hsyn+jgcamp7b/pAAV-Syn-FLEX-fd+%5BChronos-GFP%5D+(Plasmid+%2384482)/pm37585291-236-7-18
Average 91 stars, based on 1 article reviews
virus strains aav9 - by Bioz Stars, 2026-10
91/100 stars
  Buy from Supplier
aav1 aav9 hsyn dio jgcamp7b taitool cat  (Addgene inc)
96
Addgene inc aav1 aav9 hsyn dio jgcamp7b taitool cat
Figure 6. Genetic knockdown of <t>Htr1a</t> receptors in dentate gyrus (DG) impairs infraslow oscillatory (ISO) and memory performance. (A) Schematic representation of the experimental design. A mix of AAV9-CamKII-GCaMP6s and AAV1-hSyn-Cre was injected into the DG of Htr1aflox/flox mice. (B) Representative example showing photometry and EEG recordings in the DG of a control mouse injected with AAV9-CaMKII-GCaMP6s alone. Right, Fourier transformation of calcium activity during wake (blue) non-rapid eye movement (NREM) sleep (red). (C) A representative example showing
Aav1 Aav9 Hsyn Dio Jgcamp7b Taitool Cat, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aav9+hsyn+jgcamp7b/pAAV%2ESyn%2EFlex%2EGCaMP6f%2EWPRE%2ESV40+(Plasmid+%23100833)/pm40398420-296-131-142
Average 96 stars, based on 1 article reviews
aav1 aav9 hsyn dio jgcamp7b taitool cat - by Bioz Stars, 2026-10
96/100 stars
  Buy from Supplier

Image Search Results


a. Schematic of longitudinal Ca 2+ imaging recording schedules from the SoD experience session to the CFC memory recall session. b. Expression of the Janelia variant Ca 2+ indicator AAV9-hSyn-JGCaMP7b and the location of the implanted GRIN lens in the CA1 region of the dorsal hippocampus. Scale bar, 250 µm. c. Example image of cells extracted from Ca 2+ imaging and representative traces of z scored neural activity. d. Schematic of the analysis flowchart for detecting recall-specific ensembles using PCA/ICA dimensionality reduction methods. After all PCA/ICA ensembles from the CFC recall session were detected, these ensembles were further categorized into recall-specific (pink) or recall-nonspecific ensembles (blue) by comparing the ensemble activity in the CFC recall session with that in the previous CFC pre-shock session by Wilcoxon rank sum methods. For each example trace of the ensemble activity, the corresponding raster plot of the neural activity is shown with the same colour. e. Multisession comparison of the averaged activity index of the recall-specific ensembles, which is normalized by the average activity rate of the recall-nonspecific ensembles per mouse for each corresponding session. (Neutral n = 14; SoD n = 28 ensembles; two-way RM ANOVA with correction; interaction P <0.001; F (6, 240) = 5.382; Šídák’s multiple comparisons test). f. Pearson correlation analysis of the activity index of recall-specific ensemble during the SI-ICR session and prospective total freezing level (%) in the CFC recall session per SoD mouse. ( r = 0.9378, P = 0.0184, n = 5 mice) g. Activity rate of each CFC recall-specific ensemble in the neutral group in the home cage before the neutral experience (Pre-Neutral-home cage) and during the neutral session (novel home cage exploration) on day 6 ( n = 14 ensembles; Wilcoxon signed rank test; P = 0.3306); additionally, the activity rate of the SoD group in the home cage before the SoD session (Pre-SoD-home cage) and during the SoD session on day 6 of the SoD experience ( n = 28 ensembles, Wilcoxon signed rank test, P = 0.0014). h. Activity rate of each recall-specific ensemble during the novel B6 mouse encounter (SI-B6) and novel ICR mouse encounter (SI-ICR) in the social interaction test for the neutral group ( n = 14 ensembles, Wilcoxon signed rank test, P = 0.4352) and SoD group ( n = 28 ensembles, Wilcoxon signed rank test, P = 0.0066). i. Activity rate of each recall-specific ensemble during the 2-minute exploration period before receiving the first electric shock (CFC pre-shock) and during the remaining session after the delivery of the first shock (CFC post-shock) on the CFC conditioning day in the neutral group ( n = 14 ensembles; Wilcoxon signed rank test; P = 0.0076) and the SoD group ( n = 28 ensembles, Wilcoxon signed rank test, P = 0.0053). j. Schematic for assessing the correlation of the CFC recall-specific ensemble activity during opposite corner stays during the SI-ICR session and the ensemble activity during the freeze state in the CFC recall session. Example traces of averaged recall-specific ensemble activity (z scored) around the onset of the opposite corner entries in the SI-ICR (lower left) and during freezing behaviours in CFC recall (lower right) sessions. k. Spearman correlation ( r value shown) for the activity of the CFC recall-specific ensembles in the neutral group during opposite corner stays and activity during freezing in the CFC recall session for the neutral group ( r = - 0.3600, P = 0.2047) and l. SoD group ( r = 0.4426, P = 0.0208, n = 27 ensembles). m. Representative heatmaps showing the intercell type pairwise correlation analysis between SI avoidance-related (SI opposite corner-related neurons) and CFC aversion-related (shock- and freeze-related neurons) cells in the non-freezing state (left) and the freezing state in the CFC recall session (right). Red indicates a significant correlation between two neurons. n. Percentage of significant pairwise correlations of SI avoidance-related and CFC aversion-related cells during the freezing state in the CFC recall session (Neutral n = 5; SoD n = 5 mice; unpaired t test, t = 2.653; P = 0.0291). o. Percentage of significant intracell type neuron‒neuron pairwise correlations of SI avoidance-related neurons during the freezing state in the CFC recall session (Neutral, n = 5; SoD, n = 5 mice; Unpaired t test, t = 0.2808; P = 0.786). Data are presented as the mean ± s.e.m. Statistical significance is expressed as * P < 0.05; ** P < 0.01; and *** P < 0.001.

Journal: bioRxiv

Article Title: Sleep neural code perpetuates the evolving negativity bias under stress

doi: 10.64898/2026.03.03.709197

Figure Lengend Snippet: a. Schematic of longitudinal Ca 2+ imaging recording schedules from the SoD experience session to the CFC memory recall session. b. Expression of the Janelia variant Ca 2+ indicator AAV9-hSyn-JGCaMP7b and the location of the implanted GRIN lens in the CA1 region of the dorsal hippocampus. Scale bar, 250 µm. c. Example image of cells extracted from Ca 2+ imaging and representative traces of z scored neural activity. d. Schematic of the analysis flowchart for detecting recall-specific ensembles using PCA/ICA dimensionality reduction methods. After all PCA/ICA ensembles from the CFC recall session were detected, these ensembles were further categorized into recall-specific (pink) or recall-nonspecific ensembles (blue) by comparing the ensemble activity in the CFC recall session with that in the previous CFC pre-shock session by Wilcoxon rank sum methods. For each example trace of the ensemble activity, the corresponding raster plot of the neural activity is shown with the same colour. e. Multisession comparison of the averaged activity index of the recall-specific ensembles, which is normalized by the average activity rate of the recall-nonspecific ensembles per mouse for each corresponding session. (Neutral n = 14; SoD n = 28 ensembles; two-way RM ANOVA with correction; interaction P <0.001; F (6, 240) = 5.382; Šídák’s multiple comparisons test). f. Pearson correlation analysis of the activity index of recall-specific ensemble during the SI-ICR session and prospective total freezing level (%) in the CFC recall session per SoD mouse. ( r = 0.9378, P = 0.0184, n = 5 mice) g. Activity rate of each CFC recall-specific ensemble in the neutral group in the home cage before the neutral experience (Pre-Neutral-home cage) and during the neutral session (novel home cage exploration) on day 6 ( n = 14 ensembles; Wilcoxon signed rank test; P = 0.3306); additionally, the activity rate of the SoD group in the home cage before the SoD session (Pre-SoD-home cage) and during the SoD session on day 6 of the SoD experience ( n = 28 ensembles, Wilcoxon signed rank test, P = 0.0014). h. Activity rate of each recall-specific ensemble during the novel B6 mouse encounter (SI-B6) and novel ICR mouse encounter (SI-ICR) in the social interaction test for the neutral group ( n = 14 ensembles, Wilcoxon signed rank test, P = 0.4352) and SoD group ( n = 28 ensembles, Wilcoxon signed rank test, P = 0.0066). i. Activity rate of each recall-specific ensemble during the 2-minute exploration period before receiving the first electric shock (CFC pre-shock) and during the remaining session after the delivery of the first shock (CFC post-shock) on the CFC conditioning day in the neutral group ( n = 14 ensembles; Wilcoxon signed rank test; P = 0.0076) and the SoD group ( n = 28 ensembles, Wilcoxon signed rank test, P = 0.0053). j. Schematic for assessing the correlation of the CFC recall-specific ensemble activity during opposite corner stays during the SI-ICR session and the ensemble activity during the freeze state in the CFC recall session. Example traces of averaged recall-specific ensemble activity (z scored) around the onset of the opposite corner entries in the SI-ICR (lower left) and during freezing behaviours in CFC recall (lower right) sessions. k. Spearman correlation ( r value shown) for the activity of the CFC recall-specific ensembles in the neutral group during opposite corner stays and activity during freezing in the CFC recall session for the neutral group ( r = - 0.3600, P = 0.2047) and l. SoD group ( r = 0.4426, P = 0.0208, n = 27 ensembles). m. Representative heatmaps showing the intercell type pairwise correlation analysis between SI avoidance-related (SI opposite corner-related neurons) and CFC aversion-related (shock- and freeze-related neurons) cells in the non-freezing state (left) and the freezing state in the CFC recall session (right). Red indicates a significant correlation between two neurons. n. Percentage of significant pairwise correlations of SI avoidance-related and CFC aversion-related cells during the freezing state in the CFC recall session (Neutral n = 5; SoD n = 5 mice; unpaired t test, t = 2.653; P = 0.0291). o. Percentage of significant intracell type neuron‒neuron pairwise correlations of SI avoidance-related neurons during the freezing state in the CFC recall session (Neutral, n = 5; SoD, n = 5 mice; Unpaired t test, t = 0.2808; P = 0.786). Data are presented as the mean ± s.e.m. Statistical significance is expressed as * P < 0.05; ** P < 0.01; and *** P < 0.001.

Article Snippet: For longitudinal in vivo Ca2+ imaging experiments, we used a recombinant adeno-associated virus (AAV) vector, AAV9-hSyn-jGCaMP7b (Janelia variant, Addgene: #104489, viral titer: 9.77 × 1013 vg/mL).

Techniques: Imaging, Expressing, Variant Assay, Activity Assay, Comparison

Journal: Cell reports

Article Title: Synaptic zinc potentiates AMPA receptor function in mouse auditory cortex

doi: 10.1016/j.celrep.2023.112932

Figure Lengend Snippet:

Article Snippet: Injection pipettes were backfilled with mineral oil (Sigma) and filled with AAV9-hSyn1-FLEX-jGCaMP7b, retro-AAV-hSyn1-jGCaMP7b, or retro-AAV-hSyn1-GCaMP6s (titer 5e 12 – 5e 13 genome copies/mL, Addgene).

Techniques: Virus, Recombinant, Software

Figure 6. Genetic knockdown of Htr1a receptors in dentate gyrus (DG) impairs infraslow oscillatory (ISO) and memory performance. (A) Schematic representation of the experimental design. A mix of AAV9-CamKII-GCaMP6s and AAV1-hSyn-Cre was injected into the DG of Htr1aflox/flox mice. (B) Representative example showing photometry and EEG recordings in the DG of a control mouse injected with AAV9-CaMKII-GCaMP6s alone. Right, Fourier transformation of calcium activity during wake (blue) non-rapid eye movement (NREM) sleep (red). (C) A representative example showing

Journal: eLife

Article Title: Serotonin modulates infraslow oscillation in the dentate gyrus during Non-REM sleep

doi: 10.7554/elife.100196

Figure Lengend Snippet: Figure 6. Genetic knockdown of Htr1a receptors in dentate gyrus (DG) impairs infraslow oscillatory (ISO) and memory performance. (A) Schematic representation of the experimental design. A mix of AAV9-CamKII-GCaMP6s and AAV1-hSyn-Cre was injected into the DG of Htr1aflox/flox mice. (B) Representative example showing photometry and EEG recordings in the DG of a control mouse injected with AAV9-CaMKII-GCaMP6s alone. Right, Fourier transformation of calcium activity during wake (blue) non-rapid eye movement (NREM) sleep (red). (C) A representative example showing

Article Snippet: Reagent type (species) or resource Designation Source or reference Identifiers Additional information Strain (C57BL/6 J) C57BL/6 J mouse JAX: #000664 RRID:IMSR_JAX:000664 Strain (Dock10Cre) Dock10Cre mouse MGI:6117432 N/A transgene insertion, by Susumu Tonegawa Strain (Drd2Cre) Drd2Cre mouse GENSAT MMRRC:032108- UCD Strain (Slc6a4Cre) Slc6a4Cre mouse JAX: #014554 RRID:IMSR_JAX:014554 Common name: SERT- Cre Strain (Htr1aflox/flox) Htr1aflox/flox mouse Rene Hen at Columbia University N/A Generated by Rene Hen Strain (pAAV.Syn.Flex.GCaMP6s.WPRE.SV40) AAV1- SYN- FLEX- GCaMP6s Addgene RRID:Addgene_100845 Strain (AAV.CamKII.GCaMP6s.WPRE.SV40) AAV9- CamKIIa- GCaMP6s Addgene RRID:Addgene_107790 Strain (AAVdj- syn- jGCaMP7b) AAVdj- syn- jGCaMP7b Gene Vector and Virus Core at Stanford University RRID:Addgene_104489 Custom- made at Stanford virus core Strain (AAV9- hSyn- GRAB5- HT2h) AAV9- hSyn- GRAB5- HT2h Vigene Biosciences Cat#YL10097- AV9 Strain (pENN.AAV.hSyn.Cre.WPRE.hGH) AAV1- hSyn- Cre Addgene RRID:Addgene_105553 Strain (pENN.AAV.CamKII.HI.GFP- Cre.WPRE.SV40) AAV9- CaMKII- Cre- GFP Addgene RRID:Addgene_105551 Strain (pENN.AAV.CamKII0.4.eGFP.WPRE.rBG) AAV9- CaMKII- GFP Addgene RRID:Addgene_105541 Commercial assay (Htr1a RNAscope probe) Htr1a mouse RNAscope probe Advanced Cell Diagnostics Cat#312301 Commercial assay (EGFP RNAscope probe) EGFP RNAscope probe Advanced Cell Diagnostics Cat#400281

Techniques: Knockdown, Injection, Control, Transformation Assay, Activity Assay